New rare earth terbium complex synthesis and application of fluorescent probes

Title: New rare earth terbium complex synthesis and application of fluorescent probes
Author: Chen Jin snow
Degree-granting unit: Dalian University of Technology
Key words: terbium complex;; fluorescent probe;; time-resolved fluorescence analysis;; biological imaging
Summary:
Long-life fluorescent probes based on rare-earth fluorescence properties of the developed high-sensitivity chemical and biological analysis of time-resolved fluorescence detection technology has been in clinical and biological technologies have been widely used. In this paper the study, design, synthesis and characterization of three respectively for the detection of Zn2, Magnetic lifter hydroxyl radical (-OH), H_2O_2 the terbium complex fluorescent probe; N, N, N1, N1-{2, 6-- bis (3'-aminopropyl-methyl-1 '- pyrazolate) -4 - [N, N-bis (2 - pyridylmethyl) aminomethyl pyridine]} tetra-acetic acid-Tb3 (BBATA-Tb3), N, N, N1, N1-[2,6 - bis (3'-aminopropyl-methyl-1 '- pyrazol-yl) -4 - (4'-aminophenoxy) methyl pyridine] tetra-acetic acid-Tb3 (BMPTA-Tb3) and N, N, N1, N1-[2,6 - bis (3'-aminopropyl-methyl-1 '- pyrazol-yl) -4 - (3', 4'-aminophenoxy ) methyl pyridine] tetra-acetic acid-Tb3 (BMTA-Tb3). Using NMR, elemental analysis and mass spectrometry by means of synthetic intermediates and final products were characterized by the establishment of terbium complexes based on the detection of Zn2, · OH, H202 of the time-resolved fluorescence measurement techniques and time-resolved fluorescence imaging technique.
Three kinds of fluorescent probe itself is very weak, when the target substance detecting fluorescence quantum yield of reaction was significantly enhanced, as strong fluorescent complexes in aqueous solution can be achieved Zn2, · OH, H202 high-sensitivity detection. Which, BMTA-Tb3 detection of H2O2 in the horseradish peroxidase (HRP)-catalyzed achieve. http://www.999magnet.com/products/131-magnetic-lifter Probes with long fluorescence lifetime, good water solubility, good stability, high sensitivity, suitable for use under physiological conditions. After the probe is esterified through thin 'membrane into the cell, combined with time-resolved fluorescence microscopy imaging techniques, can effectively eliminate the short-lived background fluorescence from the scattered light and interference to achieve a living HeLa cells Zn2, · OH, H202 determination. Established a target in living cells highly sensitive detection method.
Degree Year: 2010