Preparation of a new terbium complex and time-resolved fluorescence imaging measurement

Title: Preparation of a new terbium complex and time-resolved fluorescence imaging measurement Author: Fu Xiaoyan Degree-granting unit: Dalian University of Technology Key words: terbium complex;; biomarkers;; time-resolved fluorescence biological imaging;; biochemical Summary: Based on long-lived fluorescence rare earth complexes developed characteristics of high-sensitivity time-resolved fluorescence analysis of biochemical and clinical testing technology has been in the field of biotechnology has been widely used, but the ideal of the existing number of rare earth complexes is very limited, severely limited the time Biochemical analysis resolved fluorescence technology. The design work, preparation and characterization of two new Tb (Ⅲ) fluorescent complexes, and to explore its biological markers and determination of time-resolved fluorescence imaging applications. Design synthesis of a carboxylic acid structure Magnetic lifter containing 5 Tb (Ⅲ) fluorescent complexes: N, N, N1, N1-[2,6 - bis (3'-aminomethyl-1 'pyrazolate) -4 - carboxy-pyridine] tetracarboxylic acid Tb (Ⅲ) (referred to as the BCTA-Tb3 +), examined the BCTA and the formation of complexes with terbium fluorescence properties, BCTA-Tb3 + fluorescence quantum yield of 8.8%, the fluorescence lifetime of 2.67 ms, while of pH fluorescence intensity and fluorescence lifetime of its effects, determine the BCTA-Tb3 + for time-resolved fluorescence measurement of the minimum detection limit of 3.64 × 10-13mol / L, show that the new terbium complexes in high sensitivity time-resolved Biochemical analysis of the fluorescence has a good prospect. Synthesis of a new type containing aldehyde methyl group Tb (Ⅲ) fluorescent complexes: N, N, N1, N1-[2,6 - bis (3'-aminopropyl-methyl-1 '- pyrazolate) -4 - aldehyde pyridine] tetracarboxylic acid Tb (Ⅲ) (referred to as BCPTA-Tb3 +), and the use of nuclear magnetic resonance spectroscopy, elemental analysis and its intermediates on BCPTA structure was characterized, from the rare earth fluorescent complexes with BCPTA luminescence mechanism triplet energy level of the experimental results discussed BCPTA-Tb3 + luminescence reason, the complex is not only a high fluorescence intensity, fluorescence lifetime long, good water solubility, etc., but also very easy to tag biological molecules with the active aldehyde groups that The new terbium complex as a very fast and efficient application of marker value. Use of BCTA-Tb3 +, BCPTA-Tb3 + as http://www.999magnet.com/products/131-magnetic-lifter a fluorescence probe labeled streptavidin avidin, respectively, for the determination of time-resolved fluorescence imaging analysis of pathogenic micro-organisms Giardia lamblia and Cryptosporidium Cryptosporidium parvum, time-resolved fluorescence immunoassay analysis showed that the use of time-resolved Fluorescence microscopy analysis, samples can be completely eliminated in the complex background of strong fluorescence interference, to achieve the ultra-complex samples Giardia lamblia and Cryptosporidium Cryptosporidium parvum high sensitivity, high specificity of detection. Degree Year: 2010