Coking wastewater activated sludge microorganisms to strengthen research capabilities

Title: Coking wastewater activated sludge microorganisms to strengthen research capabilitiesAuthor: Sheng ZhouDegree-granting units: South China University of TechnologyKey words: rare earth lanthanum chloride salt (LaCl_3 · 3H_2O);; uncultured microorganisms;; activator;; BIOLOG micro-plate technology;; PCR-DGGE;; function microorganismsSummary:

In order to quickly and accurately activated sludge from coking wastewater microbial screening of some functions and some can activate the function of activated sludge microbial activators; the same time to explore the function of coking wastewater neodymium magnets activated sludge biological treatment of wastewater microbial enhanced role. Research by the following two kinds of new ways that PCR-DGGE as an aid to screening methods, improved functional BIOLOG microbial micro-plate, and some were able to activate the function of activated sludge microbial activator for screening and research:

1 plate after separation by conventional phenol enrichment and screening of solid by beef extract peptone medium, Gao 1 solid medium, completely solid medium (TYG culture medium) TYG plate directly and through PCR-DGGE separation theory identification. Results of screening to two A1, C1 is the main function of in situ microbial; two B1, D1 is the main function of in situ microbial Africa; 5 A, B, C, D, F1 function for the in situ microbial Africa. The test results show that after the: in the activated sludge bacteria were dosing the target function A1, B1, C1, D1, E1 to strengthen the system within the COD of 600 mg / L, respectively, of the coking wastewater by 40 h, 48 h, 32 h, 40 h, 48 h of aeration, COD were reduced to 128 mg / L, 160 mg / L, 118 mg / L, 156 mg / L, 170 mg / L after stable. When dosing activated sludge were non-functional target bacteria F1, A, B, C, D intensive, the coking wastewater aeration, respectively, after 56 h, COD were reduced to 218 mg / L, 216 mg / L, 215 mg / L, 219 mg / L, 217 mg / L after the stable, the results of degradation experiments with the control group is consistent. The results showed that: the main function of activated sludge bacteria dosing of activated sludge biological treatment of the strengthening effect is most obvious; non-essential function of dosing bacteria from enhanced biological treatment of wastewater ineffective, as the main function of the activity of sewage bacteria mud enhanced significantly. Non-functional bacteria dosing of basic biological wastewater treatment http://www.chinamagnets.biz/Neodymium/Ball-Neodymium-Magnets.php without any reinforcement. Thus, by PCR-DGGE for microbial adjunct filtering is a new and effective screening method that can map from the PCR-DGGE of directly on pre-judge the activated sludge from the original habitat of microorganisms are screened for the original activated sludge microbial habitats in the function, to avoid non-conventional methods of screening to the main function of micro-organisms or non-functional micro-organisms.

(2) study via a modified BIOLOG micro-plate technology from microbial growth and reproduction of the necessary growth factors (amino acids, bases), vitamins, a large number of elements, trace elements in the filter. Results of screening to six kinds of coking wastewater activated sludge activator. Subsequently by PCR-DGGE and molecular evolutionary tree after being activated activator function of microbial strains for analysis that γ-Ami, VB12, L-Pro, DL-Ala, respectively activator most likely activated sludge Rhodococcus erythropolis, Alcaligenes faecalis, Uncultured Pseudomonas sp., Pseudomonas sp. phenol degradation function of bacteria activation; and activator A, L-His were most likely activator of activated sludge Micrococcus Sp., Uncultured Bacillus sp. non-functional phenol degradation bacteria active role. The results show that: Biolog micro-plate screening method is simple, convenient, small footprint, low supplies, a variety of targets to be effective screening of high-throughput screening methods. Rare-earth salt is a special coking wastewater activated sludge activator. Study by PCR-DGGE, phylogenetic tree building, various doses of rare earth salts of lanthanum chloride (LaCl_3 · 3H_2O) of coking wastewater activated sludge grown in TYG medium molecular ecology studies and their biological diversity. The results show that low-dose concentration of 5 mg / L and 10 mg / L LaCl_3 · 3H_2O of coking wastewater activated sludge grown in TYG medium have a stimulating effect, more biodiversity than the control group; high dose levels 200 mg / L and 400 mg / L LaCl_3 · 3H_2O of coking wastewater activated sludge grown in TYG medium has a certain inhibitory effect, less biodiversity than the control group. Concentration of low-dose 5 mg / L and 10 mg / L LaCl_3 · 3H_2O more likely to stimulate microbial groups is not represented by cultured microorganisms Uncultured Pseudomonas sp. (AB076873), Uncultured Bacillus sp. (EF072899), Uncultured bacterium clone SR40 (DQ298294 ) and culturable microbial groups bacterium rj11.Degree Year: 2009